This question is another way for the interviewer to assess if their position will be a good fit for you or if it will be a band-aid to repairing what you feel is not going right in your current or most recent position. When discussing your least favorite aspect of your current or most recent position, beware of complaining to the interviewer. The way you respond can completely change the vibe of the interview.
Take this question as an opportunity to shine when it comes to your ability to overcome challenges and dislikes. Describe the obstacles your previous employer offered you and how you thrived in that environment. Stay away from company gossip or drama. Avoid getting into a negative spiral from which you cannot recover!
When discussing your favorite aspect of your current/most recent role, stay away from company perks and choose to focus on something like growth, the culture, or your ability to make an impact. For example, avoid saying you enjoyed working at Company ABC because of the fantastic cafeteria. That's not why you worked there, and it's probably not why you're leaving.