Different interviewers will ask you different types of questions. If you are interviewing with an HR representative, you are likely to be asked about your background, experience, career aspirations, and the types of environments you thrive in. These are general questions meant to determine how well you will fit into the company's culture. Departmental hiring managers are more likely to ask you technical questions directly related to the functions you will be performing and the job you will be doing. You need to be prepared for both of these in order to have a successful interview.