The hiring authority at Union Pacific Railroad Company is looking for specifics of your job performance, leaning on recent reviews from your latest job. This question is another way of asking 'What are your strengths and weaknesses?' If you did not have formal performance reviews in your most recent position; discuss the feedback that you consistently received from your supervisors or colleagues.
Your positive highlight can be big or small! When you mention one area for improvement, avoid mentioning something that would make you ineligible for the position. For instance, if you are interviewing for an engineering role, you should not say that you are disorganized or have trouble remembering important details. Also, be sure to discuss that you are actively working to improve this weakness.